PAPER: mtDNA polymorphism affects mouse sperm ...

mtDNA polymorphism and metabolic inhibition affect sperm performance in conplastic mice

July 2017

Tourmente M, Hirose M, Ibrahim S, Dowling DK, Tompkins DM, Roldan ERS, Gemmell NJ 2017. mtDNA polymorphism and metabolic inhibition affect sperm performance in conplastic mice. Reproduction 154(4): 341-354.

ABSTRACT

Although there is a general correlation between nucleotide substitutions in the mitochondrial genome (mtDNA) and a variety of metabolic pathologies, research into the impact of particular mtDNA genotypes is still ongoing. It is still unclear how crucial mitochondrial activity is in producing the adenosine triphosphate (ATP) required for sperm function. In this study, five conplastic inbred strains with identical nuclear backgrounds but different mitochondrial genomes are used to test the impact of mtDNA polymorphisms on mouse sperm performance and bioenergetics.

KEYWORDS

Adenosine Triphosphate/metabolism; DNA, Mitochondrial/genetics/metabolism; Glycolysis/drug effects/genetics; Haplotypes; Mice, Inbred C57BL; Oxidative Phosphorylation/drug effects; Phenotype; Polymorphism, Single Nucleotide; Species Specificity; Sperm Count; Sperm Motility/drug effects; Spermatozoa/drug effects/metabolism; Uncoupling Agents/pharmacology;