Report: A. psidii biotype differential ...

Kalamorz F, Soewarto J, Frampton R, Smith G. 2024. RA1B-MR Diagnostics: Development and deployment of an Austropuccinia psidii biotype differential diagnostic test. A Plant & Food Research report prepared for: Biological Heritage. Milestone No. 90988. Contract No. 39454. Job code: P/313069/01. PFR SPTS No. 25282. 21 p.

Five Loop Mediated Isothermal Amplification (LAMP) assays, designed by USA-based colleagues to specifically diagnose four of the genetic clusters (clades) of Austropuccinia psidii (Pandemic (two assays), Eucalyptus, Guava, South African) were tested at The New Zealand Institute for Plant and Food Research Limited (PFR), Lincoln using a plasmid with an insert containing the differential sequence targets. The LAMP assay was also tested against 12 genomic DNA preparations from fungal spores collected from infected plants in Aotearoa-New Zealand.

This report provides the findings of these assays.