PAPER: DNA aptamers for rat pest detection

Selection and characterization of DNA aptamers for the rat major urinary protein 13 (MUP13) as selective biorecognition elements for sensitive detection of rat pests

November 2021

Lucarelli V, Colbert D, Li S, Cumming M, Linklater W, Mitchell J, Travas-Sejdic J, Kralicek A. 2022. Selection and characterization of DNA aptamers for the rat major urinary protein 13 (MUP13) as selective biorecognition elements for sensitive detection of rat pests. Talanta 240: 123073.

ABSTRACT

Among invasive mammalian predators, rats represent a major threat, endangering ecosystem functioning worldwide. After rat-control operations, detecting their continued presence or reinvasion requires more sensitive and lower cost detection technologies. Here, we develop a new sensing paradigm by using a specific rat urine biomarker (MUP13) to unambiguously signal the presence of rats.

KEYWORDS

Major urinary protein; Aptamer; SELEX; Fluorescence anisotropy; Surface plasmon resonance;