PAPER: Fatty Acid Methyl Ester (FAME) profile

The Fatty Acid Methyl Ester (FAME) profile of Phytophthora agathidicida and its potential use as diagnostic tool

September 2021

Lacey RF, Sullivan-Hill BA, Deslippe JR, Keyzers RA, Gerth ML. 2021. The Fatty Acid Methyl Ester (FAME) profile of Phytophthora agathidicida and its potential use as diagnostic tool. FEMS Microbiology Letters 368(17).

ABSTRACT

In New Zealand, Phytophthora agathidicida is threatening the survival of kauri, an endemic, culturally and ecologically important tree species. The current method for detection is time-consuming and requires high levels of expertise to assess, thus limiting the analytical sample throughput. Here, we characterized the fatty acid methyl ester (FAME) profile of P. agathidicida and compared it with the FAME profile of P. cinnamomi to assess the efficacy of FAME analysis as a diagnostic tool for detecting the pathogen in soil samples.

KEYWORDS

Phytophthora; Phytophthora agathidicida; diagnostics; fatty acid methyl ester analysis