@prefix dcat: . @prefix dct: . @prefix foaf: . @prefix xsd: . a dcat:Dataset ; dct:description """#Tranche 2: Strategic Objective 4#\r \r ##Automation can improve biosecurity surveillance systems##\r \r Surveillance is an essential part of protecting New Zealand’s economic assets and natural taonga from damaging exotic organisms.\r \r Our government currently spends over $125 million a year on monitoring for biological threats. It’s an expensive process because it requires thousands of hours of highly skilled human labour.\r \r As part of the BioHeritage National Science Challenge the State-of-the-art Surveillance team is developing prototype technologies that will automate and improve surveillance results, while saving costs.\r \r ###Research Area Summary###\r \r **Spectral imaging for urban tree health**\r \r Our research team is using a camera mounted on a moving vehicle to monitor the health of our urban rākau (trees).\r \r The camera takes pictures of street trees every fortnight. Images are pre-processed to maintain people’s privacy (blurring cars, people, houses etc.) and then analysed by computer to identify what kind of trees each street has, and whether they look damaged by pest or disease.\r \r When we find sick trees we can send a biosecurity inspector to focus just on the damaged trees – saving time and money. It works the other way, too: if our DNA project above discovers a new insect has entered the country, and we know that it’s a threat to a specific kind of tree, we’ll have a map of where those trees are in our cities and can go inspect them.\r \r **Insect Soup: sampling eDNA with light traps**\r \r Our researchers are installing UV light traps at the Port of Tauranga to help detect the arrival of foreign insects.\r \r All of the insects caught will be analyzed in the lab by a process called High-Throughput Sequencing (HTS). This will tell us the DNA of every insect species caught in the trap.\r \r If a new insect species or a known threat comes into Tauranga, we know where and when it was collected, and we can take steps to eradicate it.\r \r Developing DNA diagnostic technologies like this allows us to cost-effectively scale-up our efforts to find new pests early. This early detection is essential for protecting our natural and productive environments.\r \r **Leader:** \r \r - Steve Pawson (University of Canterbury)""" ; dct:identifier "d365efb7-3152-4498-bd0f-1d82392ae237" ; dct:issued "2024-01-16T22:34:18.330117"^^xsd:dateTime ; dct:modified "2024-08-13T00:06:26.972635"^^xsd:dateTime ; dct:publisher ; dct:title "State-of-the-Art Surveillance" ; dcat:distribution , , , , , , , , , , . a dcat:Distribution ; dct:description """###Online quick-fire presentation from Crazy & Ambitious 4 (Day 1): Te Tiriti-guided national DNA reference library\r \r **April 2024**\r \r Online quick-fire presentation from Crazy & Ambitious 4: Te Tiriti-guided national DNA reference library, with Manpreet Dhami, Maanaki Whenua Landcare Research.""" ; dct:issued "2024-08-12T03:10:15.013595"^^xsd:dateTime ; dct:modified "2024-08-12T03:10:15.013595"^^xsd:dateTime ; dct:title "WEBINAR: C&A4 Te Tiriti-guided DNA library " ; dcat:accessURL . a dcat:Distribution ; dct:description """###Multi-trap sampling of arthropod communities at ports of first entry informs biosecurity surveillance programs###\r \r **December 2023**\r \r **Wardhaugh CW, Pawson SM 2023. [Multi-trap sampling of arthropod communities at ports of first entry informs biosecurity surveillance programs](https://link.springer.com/content/pdf/10.1007/s10340-023-01714-5.pdf). Journal of Pest Science. **\r \r **ABSTRACT**\r \r Ports of first entry are high-risk locations where invasive species may arrive, establish, and cause wider potential environmental, economic, social, or cultural harm. Early detection provides the opportunity to eradicate or limit the spread and scale of any harm. Most surveillance operations target specific high-profile pests, thus missing many potentially invasive species. We compared eight surveillance trap types at a New Zealand port to determine optimal trap types and trapping intensity to adequately sample a broad spectrum of the invertebrate fauna to inform future biosecurity surveillance networks. We collected 253,582 invertebrates in total, including 46,819 beetles that were sorted to 209 morphospecies (excluding Staphylinidae) for species-level analyses. One individual of a species (*Cryptarcha* sp. indet., Coleoptera: Nitidulidae) previously unknown from New Zealand was collected by a UV light trap. UV light traps collected the largest proportion (84.3%) of beetle species compared to other trap types (range 3–38%). Species overlap was high between UV light traps and traps that collected flying beetles (range 82.3–92.4% for flight intercept and Malaise traps), but overlap was lower with pitfall traps (62.2%). Trapping intensity of ~ 300 weekly samples (15 UV light traps and 30 pitfall traps operating for eight non-sequential weeks) would sample ~ 250 (83%) of the ~ 300 beetle species on the port. We recommend UV light traps in particular for surveillance on ports and other discrete high-risk areas due to their broad-spectrum attractiveness to flying insects. Since diagnostics is an impediment to processing species-rich samples, the establishment of an operational surveillance network of UV light traps will likely require eDNA metabarcoding techniques.\r \r **KEYWORDS**\r \r Biological invasions;\r Coleoptera;\r General surveillance;\r Insect trapping;\r Pathway analysis;\r Risk assessment""" ; dct:issued "2024-01-17T01:55:49.246347"^^xsd:dateTime ; dct:modified "2024-01-17T01:55:49.246347"^^xsd:dateTime ; dct:title "PAPER: Multi-trap sampling of arthropod" ; dcat:accessURL . a dcat:Distribution ; dct:description """###State-of-the-art Surveillance###\r \r **To view this website: ** [CLICK ON THIS LINK](https://bioheritage.nz/research/state-of-the-art-surveillance/)\r \r **NOTE:** This website will cease in 2027""" ; dct:format "PDF" ; dct:issued "2024-01-17T03:13:11.904747"^^xsd:dateTime ; dct:modified "2024-01-17T03:13:11.904747"^^xsd:dateTime ; dct:title "WEBPAGE: SO4" ; dcat:accessURL ; dcat:byteSize 364327.0 ; dcat:mediaType "application/pdf" . a dcat:Distribution ; dct:description """###Online presentation from Crazy & Ambitious 4 (Day 2): Te Tiriti-guided national DNA reference library###\r \r **April 2024**\r \r Online presentation from Crazy & Ambitious 4: Te Tiriti-guided national DNA reference library, with Manpreet Dhami, from Manaaki Whenua Landcare Research. \r \r Part of the presentation, Co-design for community surveillance, with Te Poari Newton, Stevee Raureti, Brayden Hohaia, and Serene Ratu, from Kāwai Kaimai.""" ; dct:issued "2024-08-12T23:51:13.823665"^^xsd:dateTime ; dct:modified "2024-08-12T23:51:13.823665"^^xsd:dateTime ; dct:title "WEBINAR: C&A4 Te Tiriti-guided national DNA reference library" ; dcat:accessURL . a dcat:Distribution ; dct:description """###Crazy and Ambitious 3: Session 3 - State-Of-The-Art Surveillance###\r \r **May 2022**\r \r **Presentations from State-Of-The-Art Surveillance:**\r \r 1) Tama Blackburn (Ngāti Maniapoto; Taranaki Mounga)\r \r 2) Richard Green (University of Canterbury)\r \r 3) Manpreet Dhami (Manaaki Whenua - Landcare Research)\r \r Followed by guest speaker:** David Teulon (Better Border Biosecurity)""" ; dct:issued "2024-01-17T20:42:43.485911"^^xsd:dateTime ; dct:modified "2024-01-17T20:42:43.485911"^^xsd:dateTime ; dct:title "WEBINAR: C&A3 State-Of-The-Art Surveillance" ; dcat:accessURL . a dcat:Distribution ; dct:description """###Find-A-Pest: Catch it, snap it, report it!###\r \r **March 2024**\r \r How can we support early detection of new pests and increase our understanding of the distribution and density of existing pests? Through harnessing citizen science!\r """ ; dct:format "PDF" ; dct:issued "2024-03-29T21:09:33.780133"^^xsd:dateTime ; dct:modified "2024-03-29T21:09:33.780133"^^xsd:dateTime ; dct:title "BRIEF: FIND-A-PEST" ; dcat:accessURL ; dcat:byteSize 12450466.0 ; dcat:mediaType "application/pdf" . a dcat:Distribution ; dct:description """###Online quick-fire presentation from Crazy & Ambitious 4: Surveillance - new tools and technologies for surveillance###\r \r **April 2024**\r \r Online quick-fire presentation from Crazy & Ambitious 4: Surveillance - new tools and technologies for surveillance, with Steve Pawson.""" ; dct:issued "2024-08-12T02:49:44.342072"^^xsd:dateTime ; dct:modified "2024-08-12T02:49:44.342072"^^xsd:dateTime ; dct:title "WEBINAR: C&A4 New Surveillance Tools & Tech" ; dcat:accessURL . a dcat:Distribution ; dct:description """###Fast-tracking bespoke DNA reference database generation from museum collections for biomonitoring and conservation###\r \r **November 2022**\r \r **Dopheide A, Brav-Cubitt T, Podolyan A, Leschen RAB, Ward D, Buckley TR, Dhami MK 2022. [Fast-tracking bespoke DNA reference database generation from museum collections for biomonitoring and conservation](https://onlinelibrary.wiley.com/doi/epdf/10.1111/1755-0998.13733). Molecular Ecology Resources**\r \r **ABSTRACT**\r \r Despite recent advances in high-throughput DNA sequencing technologies, a lack of locally relevant DNA reference databases limits the potential for DNA-based monitoring of biodiversity for conservation and biosecurity applications. Museums and national collections represent a compelling source of authoritatively identified genetic material for DNA database development, yet obtaining DNA barcodes from long-stored specimens may be difficult due to sample degradation. Here we demonstrate a sensitive and efficient laboratory and bioinformatic process for generating DNA barcodes from hundreds of invertebrate specimens simultaneously via the Illumina MiSeq system. Using this process, we recovered full-length (334) or partial (105) COI barcodes from 439 of 450 (98%) national collection-held invertebrate specimens. This included full-length barcodes from 146 specimens which produced low-yield DNA and no visible PCR bands, and which produced as little as a single sequence per specimen, demonstrating high sensitivity of the process. In many cases, the identity of the most abundant sequences per specimen were not the correct barcodes, necessitating the development of a taxonomy-informed process for identifying correct sequences among the sequencing output. The recovery of only partial barcodes for some taxa indicates a need to refine certain PCR primers. Nonetheless, our approach represents a highly sensitive, accurate and efficient method for targeted reference database generation, providing a foundation for DNA-based assessments and monitoring of biodiversity.\r \r **KEYWORDS**\r \r conservation; DNA-based monitoring; invertebrate barcoding; molecular taxonomy; museum collection; taxonomy-informed bioinformatics pipeline""" ; dct:issued "2024-01-16T22:39:57.476943"^^xsd:dateTime ; dct:modified "2024-01-16T22:39:57.476943"^^xsd:dateTime ; dct:title "PAPER: DNA Database: Museums Biomonitoring" ; dcat:accessURL . a dcat:Distribution ; dct:description """###Evaluating New Technology for Biosecurity Surveillance###\r \r **March 2024**\r \r Biosecurity surveillance helps prevent the establishment of, control, and eradication of unwanted and damaging organisms in Aotearoa New Zealand. Existing surveillance either relies on the public to call in potential threat or is conducted through programmes focused on a particular species, group, or pathway of entry.\r \r We investigated innovative technology that could be used to detect a broader range of unwanted organisms within a single programme.\r """ ; dct:format "PDF" ; dct:issued "2024-03-28T03:25:51.692262"^^xsd:dateTime ; dct:modified "2024-03-28T03:25:51.692262"^^xsd:dateTime ; dct:title "BRIEF: Evaluating New Technology for Biosecurity Surveillance" ; dcat:accessURL ; dcat:byteSize 7400769.0 ; dcat:mediaType "application/pdf" . a dcat:Distribution ; dct:description """###Online presentation from Crazy & Ambitious 4: Co-design for community surveillance###\r \r **April 2024**\r \r Online presentation from Crazy & Ambitious 4: Co-design for community surveillance, with Te Poari Newton, Stevee Raureti, Brayden Hohaia, and Serene Ratu, from Kāwai Kaimai.\r \r Includes presentation: Te Tiriti-guided national DNA reference library, with Manpreet Dhami, from Manaaki Whenua Landcare Research """ ; dct:issued "2024-08-12T22:52:00.735453"^^xsd:dateTime ; dct:modified "2024-08-12T22:52:00.735453"^^xsd:dateTime ; dct:title "WEBINAR: C&A4 Co-design for community surveillance" ; dcat:accessURL . a dcat:Distribution ; dct:description """###State-Of-The-Art Surveillance###\r \r **December 2021**\r \r **ABSTRACT**\r \r Surveillance is an essential part of protecting New Zealand’s economic assets and natural taonga from damaging exotic organisms. To strengthen our biosecurity system, researchers at BioHeritage are investigating two technologies to improve the early detection of new invaders. Dr Steve Pawson explains more:\r \r Insect Soup: sampling DNA with light traps\r Our team is installing UV light traps at the Port of Tauranga to help detect the arrival of foreign insects. All of the insects caught will be analysed in the lab by a process called High-Throughput Sequencing (HTS). This will tell us the DNA of every insect species caught in the trap. If a new insect species or a known threat comes into Tauranga, we know where and when it was collected, and we can take steps to eradicate it.\r \r Spectral imaging for urban tree health\r Our researchers are trialing cameras mounted on moving vehicles to monitor the health of our urban rākau (trees). The camera will take pictures of street trees every fortnight. Images will be pre-processed to maintain people’s privacy (blurring cars, people, houses etc.) and then analysed by computer to identify what kind of trees each street has, and whether they look damaged by pests or diseases.\r \r PRESENTER: Dr Stephen Pawson, School of Forestry, University of Canterbury.\r """ ; dct:issued "2024-01-22T02:01:21.147535"^^xsd:dateTime ; dct:modified "2024-01-22T02:01:21.147535"^^xsd:dateTime ; dct:title "WEBINAR: State-of-the-art Surveillance" ; dcat:accessURL . a foaf:Organization ; foaf:name "Challenge Inventory" .