PAPER: Variability and Uncertainty in eDNA Data
URL: https://doi.org/10.3389/fevo.2020.00135
A Systematic Review of Sources of Variability and Uncertainty in eDNA Data for Environmental Monitoring
May 2020
Mathieu C, Hermans SM, Lear G, Buckley TR, Lee KVC, Buckley HL 2020. A Systematic Review of Sources of Variability and Uncertainty in eDNA Data for Environmental Monitoring. Frontiers in Ecology and Evolution 8(135).
ABSTRACT
Environmental DNA (eDNA) is becoming a standard tool in environmental monitoring that aims to quantify spatiotemporal variation for the measurement and prediction of ecosystem change. eDNA surveys have complex workflows encompassing multiple decision-making steps in which uncertainties can accumulate due to field sampling design, molecular biology lab work, and bioinformatics analyses.
KEYWORDS
bioinformatics; community; edna; experimental design; single taxon; spatiotemporal scale; uncertainty; variability; microbial ecology; occupancy models; DNA extraction; biodiversity; diversity; bacterial; communities; abundance; scale; sensitivity;
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